Electronic Journal of Differential Equations, Vol. 2007(2007), No. 142, pp. 1-9. Title: Ambarzumian's theorem for trees Authors: Robert Carlson (Univ. of Colorado at Colorado Springs, USA) Vyacheslav Pivovarchik (South-Ukrainian State Pedag. Univ., Ukraine) Abstract: The classical Ambarzumian's Theorem for Schrodinger operators $-D^2 + q$ on an interval, with Neumann conditions at the endpoints, says that if the spectrum of $(-D^2+q)$ is the same as the spectrum of $(-D^2)$ then $q=0$. This theorem is generalized to Schrodinger operators on metric trees with Neumann conditions at the boundary vertices. Submitted April 12, 2007. Published October 24, 2007. Math Subject Classifications: 34B45. Key Words: Inverse eigenvalue problem; quantum graph.